Surface Plasmon Resonance
From Anvita Health Wiki
Introduction
- No radioactive label.
- Smallest protein for which technique successfully employed 180,000 daltons [2003]
- ligand is attached to the sensor chip
- gold- dextran surface (maximum 400 runs on same surface)
- protein in sample binds to ligand
- changes in refractive index measured
- resonance of electrons in gold excited by red light
- ligand binding effect electron delocalization in some manner as to affect refractive index
- Gives on & off rates, kD
More General Terms
References
Biacore
