Pcr/southern Blot/northern Blot/ish For Breast Cancer

From Anvita Health Wiki

Contents 1 Indications 2 Specimen 3 Notes 4 More General Terms 5 Additional Terms 6 References

Indications

• breast cancer

Specimen

• tumor tissue
• whole blood ( ACD or EDTA)
• paraffin-embedded tissue
• store body fluid at 4 degrees C
• store tissue at -20 degrees C or below

Notes

• associated genetic aberrations:
• BRCA1
• BRCA2
• her2/neu proto-oncogene ( erb-B2)
• c-myb proto-oncogene
• c-myc proto-oncogene
• H-ras proto-oncogene
• K-ras proto-oncogene
• N-ras proto-oncogene
• wnt1 (int1) proto-oncogene
• p53 gene
• retinoblastoma [Rb] gene (Rb1)
• gene for cathepsin D
• gene for metastasis inhibition factor nm23

More General Terms

• molecular diagnostic test

Additional Terms

• breast cancer
• breast cancer type 1 susceptibility protein (BRCA1)
• breast cancer type 2 susceptibility protein (Fanconi anemia group D1 protein, BRCA2, FACD, FANCD1)
• c-myb proto-oncogene
• c-myc proto-oncogene
• cathepsin-D (CTSD, CPSD)
• H-ras proto-oncogene
• her2/neu proto-oncogene (erb-B2)
• K-ras proto-oncogene
• N-ras proto-oncogene
• nucleoside diphosphate kinase-A, (NDP kinase A, tumor metastatic process-associated protein, metastasis inhibition factor nm23, nm23-H1, NME1, nm23/H1 nucleoside diphosphate kinase, granzyme A-activated DNase, GAAD)
• p53 gene
• retinoblastoma [Rb] gene (Rb1)
• wnt1 proto-oncogene or int1 proto-oncogene

References

Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed.,  W.B. Saunders, 1995

PCR/southern blot/northern blot/in-situ hybridization for breast cancer