Nitric Oxide

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Introduction

  • A colorless gas & a free radical. NO reacts rapidly with oxygen to form nitrogen oxides, which ultimately convert to nitrite (NO2-) & nitrate (NO3-).

* NO +  O2  ->  NO2  +  N2O3  +  N2O2   ->  NO2-  +  NO3-


  • It is a gaseous mediator of cell to cell communication & a potent vasodilator. It is formed from L-arginine by:
  • a constitutuve NO synthase (NO synthase-1 or NO synthase-3) in
  • brain
  • peripheral nerves
  • endothelium (NO-synthase-3)
  • bone
  • granulocytes
  • pancreatic beta cells
  • an inducible NO synthase (NO synthase-2) in
  • Kupffer cells
  • macrophages
  • smooth muscle
  • NO activates soluble guanylate cyclase.
  • It mediates penile erection.
  • In the immune system, macrophages use NO as a cytotoxic agent.
  • Insufficient NO may contribute to hypertension.
  • Free NO in the circulation is rapidly reduced by Fe+2 of hemoglobin.
  • Nitric oxide covalently binds to cysteine residues of proteins resulting in S-nitrosylated derivatives. [2,3] S-nitrosylation is reversible.
  • Targets of S-nitrosylation include:
  • Na+ pump
  • Na+ K+ ATPase
  • alpha tubulin
  • NMDA receptor
  • GAPDH*
  • * S-nitrosylation of GAPDH triggers nuclear translocation via binding to Siah1. Nitrosylated GAPDH stablizes Siah1, an E3-ubiquitin ligage that mediates proteolysis of nuclear proteins leading to apoptosis.

More General Terms

Additional Terms

Internet Database

PubChem: 945
PubChem: 84878
PubChem: 145068
PubChem: 3001380

References

  1. Stedman's Medical Dictionary 27th ed, Williams & Wilkins, Baltimore, 1999
  2. Tidball J, 9th Annual UCLA Research Conference on Aging, June 17, 2004 (conference speaker)
  3. Sedlak TW, Snyder SH. Messenger molecules and cell death: therapeutic implications. JAMA. 2006 Jan 4;295(1):81-9. PMID: [1]
  4. National Guideline Clearinghouse NIH Consensus Development Conference statement on inhaled nitric oxide therapy for premature infants. National Institutes of Health Consensus Development Conference ngc-guideline: [2]

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