In Situ Hybridization

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Contents 1 More Specific Terms 2 Indications 3 Methods 4 Specimen 5 More General Terms 6 References

More Specific Terms

• fluorescence in situ hybridization

Indications

• detection of virus DNA or RNA in infected cells

Methods

• allows direct visualization of specific sequence of DNA or RNA at the cellular level
• sensitivity is low*
• presence of relatively large amounts of DNA or RNA is necessary for visualization
  

• * fluorescence in situ hybridization increases sensitivity

Specimen

• fixed tissue sections
• exfoliated cells (smears or cytospin preparations)
• samples must adhere to glass slides for microscopy

• Turnaround time: generally 3-7 days

More General Terms

• laboratory medicine (clinical pathology)

References

Clinical Guide to Laboratory Tests, 3rd ed. Teitz ed.,  W.B. Saunders, 1995

in situ hybridization (ISH)