Heparan Sulfate N Deacetylase/n Sulfotransferase 2

From Anvita Health Wiki

Contents 1 Function 2 Structure 3 Compartment 4 Alternative-splicing 5 Notes 6 More General Terms 7 Internet Database 8 References

Function

• essential bifunctional enzyme that catalyzes both N-deacetylation & the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate
• modifies GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis
• role in determining extent & pattern of sulfation of heparan sulfate
• glycan metabolism; heparan sulfate biosynthesis
• glycan metabolism; heparin biosynthesis

* 3'-phosphoadenylyl sulfate + [heparansulfate]-glucosamine  
*                  <-->  
* adenosine 3',5'-bisphosphate +  [heparansulfate]-N-sulfoglucosamine

• Kinetic parameters:

• KM=4.7 uM for K5 polysaccharide
• KM=0.76 uM for N-acetylated HS-II

Structure

• monomer ( putative)
• belongs to the sulfotransferase 1 family, NDST subfamily

Compartment

• Golgi membrane

Alternative-splicing

• named isoforms=2

Notes

• the presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/ sulfation reactions, whereas others later on fill in or extend already modified heparan sulfate sequences

More General Terms

• sulfotransferase
• glycoprotein
• membrane protein

Internet Database

UniProt: [1]
Entrez gene: 8509
Kegg: [2]
OMIM: 603268

References

1. UniProt [3]
2. GGDB; Glycogene database [4]

bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2; glucosaminyl N-deacetylase/N-sulfotransferase 2; NDST-2; N-heparan sulfate sulfotransferase 2; N-HSST 2 [includes: heparan sulfate N-deacetylase 2; heparan sulfate N-sulfotransferase 2] (NDST2, HSST2)