Heparan Sulfate N Deacetylase/n Sulfotransferase

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Function

  • essential bifunctional enzyme
  • catalyzes both N-deacetylation & N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate
  • modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis
  • plays a role in determining the extent & pattern of sulfation of heparan sulfate
  • role in biosynthesis of heparan sulfate that can ultimately serve as L- selectin ligands, thereby playing a role in inflammatory response
  • glycan metabolism; heparan sulfate biosynthesis
  • glycan metabolism; heparin biosynthesis 
<br>  
* 3'-phosphoadenylyl sulfate + [heparansulfate]-glucosamine 
*                <--> 
* adenosine 3',5'-bisphosphate +  [heparan sulfate]-N-sulfoglucosamine <br> 
* Kinetic parameters: 
:* KM=13.3 uM for K5 polysaccharide 
:* KM=0.35 uM for N-acetylated HS-II
== Structure ==

:* [[Monomer | monomer]] 
:* belongs to the [[Sulfotransferase | sulfotransferase]] 1 family, NDST subfamily
== Compartment ==

:* [[Golgi]] membrane s
== Alternative-splicing ==

:* named [[Isoform | isoforms]]=2
== Expression ==

:* widely expressed 
:* most abundant in heart, liver & [[Pancreas | pancreas]]
== Notes ==

:* the presence of 4 different  [[Heparan_Sulfate_N_Deacetylase | heparan sulfate N-deacetylase]]/N-[[Sulfotransferase | sulfotransferase]] [[Enzyme | enzymes]] in [[Mammal | mammals]], as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/ [[Sulfotyrosine_Site | sulfation]] reactions, whereas others later on fill in or extend already modified heparan sulfate sequences
== More General Terms ==

* [[Sulfotransferase | sulfotransferase]] 
* [[Glycoprotein | glycoprotein]] 
* [[Membrane_Protein | membrane protein]]
== Internet Database==

Entrez gene: [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=3340 3340] <br />Kegg: [http://www.genome.jp/dbget-bin/www_bget?hsa:3340] <br />OMIM: [http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600853 600853] <br />UniProt: [http://www.expasy.org/uniprot/P52848]
== References ==

#UniProt [http://www.uniprot.org/uniprot/P52848.html] 
#GGDB; Glycogene database [http://riodb.ibase.aist.go.jp/rcmg/ggdb/]
<span id="long_desc" style="display: none">bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; glucosaminyl N-deacetylase/N-sulfotransferase 1; NDST-1; [heparan sulfate]-glucosamine N-sulfotransferase 1; HSNST 1; N-heparan sulfate sulfotransferase 1; N-HSST 1 [includes: heparan sulfate N-deacetylase 1; heparan sulfate N-sulfotransferase 1] (NDST1, HSST, HSST1)</span>
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