Band 3 Protein

From Anvita Health Wiki

Contents 1 Introduction 2 Function 3 Structure 4 Compartment 5 Expression 6 Pathology 7 Polymorphism 8 More General Terms 9 Internet Database 10 References

Introduction

• Band # from SDS gel of erythrocyte ghosts.

Function

• Cl-/ HCO3- exchange
• the major integral glycoprotein of the erythrocyte membrane
• two functional domains

• integral domain mediates a 1:1 exchange of inorganic anions across the membrane, i.e.
• cytoplasmic domain provides binding sites for cytoskeletal proteins, glycolytic enzymes, & hemoglobin

• phosphorylated on Tyr-8 & Tyr-21 most likely by SYK
• PP1- resistant phosphorylation that precedes Tyr-359 & Tyr-904 phosphorylation
• phosphorylated on Tyr-359 & Tyr-904 most likely by LYN
• PP1-inhibited phosphorylation that follows Tyr-8 & Tyr-21 phosphorylation
• phenyl isothiocyanate inhibits anion transport in vitro

Structure

• a dimer in solution
• spans the membrane asymmetrically
• appears to be tetrameric
• belongs to the anion exchanger (TC 2.A.31) family

Compartment

• membrane

Expression

• erythrocytes

Pathology

• defects in SLC4A1 are the cause of:

• elliptocytosis type 4
• autosomal dominant distal renal tubular acidosis
• autosomal recessive distal renal tubular acidosis

• defects in SLC4A1 are a cause of

• hereditary spherocytosis

Polymorphism

• SLC4A1 is responsible for 3 blood groups

• Diego blood group
• Swann blood group ( SW)
• Froese blood group

More General Terms

• anion exchange protein
• Cl- channel
• PEST protein
• CD antigen
• solute carrier family 4 (SLC4, SLC4A)
• glycoprotein
• plasma membrane protein

Internet Database

Entrez gene: 6521
Kegg: [1]
OMIM: 109270
OMIM: 110500
OMIM: 112010
OMIM: 112050
OMIM: 130600
OMIM: 179800
OMIM: 601550
OMIM: 601551
OMIM: 611590
Prosite: [2]
Prosite: [3]
UniProt: [4]

References

1. Alperts et al Molecular Biology of the Cell, Garland Publishing, New York, NY 1989 pg 289-291
2. Wang KK et al Calmodulin-binding proteins as calpain substrates. Biochem J 262:693 1989 PMID: [5]
3. UniProt [6]
4. Wikipedia; Note: Band 3 entry [7]
5. BGMUT; Note: Blood group antigen gene mutation database [8]
6. GeneReviews [9]
7. SeattleSNPs [10]

band 3 anion transport protein; anion exchange protein 1; anion exchanger 1; AE 1; solute carrier family 4 member 1; CD233 (SLC4A1, AE1, DI, EPB3)